New fluorescence technique opens window to protein complexes in living cells

Fluorescent microscopy makes use of molecules, such as green fluorescent protein (GFP), that emit colored light when illuminated with light of a specific wavelength. Molecules like GFP can be used to label proteins of interest and can reveal information about the relationships of molecules within cells. Fluorescence polarization, also known as anisotropy, is specific parameter of fluorescence that can provide additional information about the properties of individual molecules.

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