Researchers develop new monomer fluorescent protein for SR imaging
Monday, August 29, 2016 - 08:31
in Physics & Chemistry
To understand the cell, it is necessary to study its dynamics at high resolution in space and time using techniques that do not adversely affect it. Recently developed superresolution (SR) microscopy breaks the diffraction limit and offers the requisite spatial resolution but usually at the cost of slow imaging speed and excessive damage. Applying reversibly switchable fluorescent proteins (RSFPs) greatly reduces the illumination intensity, thus enabling live-cell SR imaging while using saturated depletion-based SR techniques such as nonlinear structured illumination microscopy (SD NL-SIM) or reversible saturable optical fluorescence transition (RESOLFT) microscopy.
